2013 International Symposium on Ranaviruses

ranavirus conf logo 2012.jpg

Knoxville, Tennessee, USA


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Presentation Slides


Saturday, 27 July 2013


Moderator: Trent Garner


8:30 – 9:30

Keynote Address 

Richard Speare
Emeritus Professor, School of Public Health, James Cook University, Australia

9:30 – 10:00

Emergence and Conservation Overview

Trenton Garner
Institute of Zoology, Zoological Society of London

10:00 – 10:15

Ranavirus could potentially speed up extinction for the endangered frog (Rana sevosa)

Julia Earl
National Institute for Mathematical and Biological Synthesis, University of Tennessee

10:15 – 10:30

Repeated detection of frog virus 3 (FV3) during aquaculture health surveys

Thomas Waltzek
College of Veterinary Medicine, University of Florida

10:45 – 11:00

Concurrent die-offs of turtles, salamanders and frogs at one site in Maryland, USA

Scott Farnsworth
School of Biological Sciences, Washington State University

11:00 – 11:15

Distribution of ranaviruses in Japan

Yumi Une
Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University

11:15 – 11:30

Ranavirus infection in Costa Rican amphibians

Jacob Kerby
Department of Biology, University of South Dakota

11:30 – 11:45

Characterization of amphibian ranavirus in the international wildlife trade

Kristine Smith
EcoHealth Alliance


Moderator: Debra Miller


13:30 – 14:00

Pathology and Physiology Overview

Debra Miller

College of Veterinary Medicine and Center for Wildlife Health, University of Tennessee, Knoxville

14:00 – 14:15

Frog virus 3 in eastern box turtles: Agents seen with coinfections

James Wellehan
College of Veterinary Medicine, University of Florida

14:15– 14:30

Ranavirus associated dermatitis in lizards

Anke Stöhr
Institute of Environmental and Animal Hygiene, Universität Hohenheim

14:30 – 14:45

The wood frog, Rana sylvatica (Lithobates sylvaticus), as a model to study the pathogenesis and host-pathogen interactions of frog virus 3 (FV3)

Maria Forzán
Canadian Cooperative Wildlife Health Centre and Department of Pathology and Microbiology, Atlantic Veterinary College

14:45 – 15:00

Ranaviruses in snakes, lizards and chelonians

Rachel Marschang
Institute of Environmental and Animal Hygiene, Universität Hohenheim


Moderator: Jacques Robert


15:15 – 15:45

Virology and Immunology Overview

Jacques Robert
Microbiology & Immunology, University of Rochester Medical Center

15:45 – 16:00

The three dimensional structure and morphogenesis of Singapore grouper iridovirus

Jinlu Wu
Mechanobiology Institute Singapore, National University of Singapore, Singapore

16:00 – 16:15

Experimental challenge study of ranavirus infection in previously infected eastern box turtles (Terrapene carolina carolina) to assess immunity

Jennifer Hausmann
Medical Department,  Maryland Zoo

16:15 – 16:30

Immune response in fathead minnow cells following infection with frog virus 3

V. Gregory Chinchar
Department of Microbiology, University of Mississippi Medical Center

16:30 – 16.:45

Signapore grouper iridovirus (SGIV) induced parapoptosis-like death in host cells via the activation of MAPK signaling

Qiwei Qin
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences


Sunday, 28 July 2013


Moderator: Allan Pessier


8:30 – 9:00

Diagnosis, Treatment and Management Overview

Allan Pessier
Amphibian Disease Laboratory, Institute for Conservation Research, San Diego Zoo

9:00 – 9:15

Chelonian diagnostics, pathology, and therapy

Matthew Allender
Department of Comparative Biosciences, , College of Veterinary Medicine, University of Illinois

9:15 – 9:30

Monitoring ranavirus-associated mortality in Dutch heathland in the aftermath of an outbreak

Annemarieke Spitzen
Reptile, Amphibian & Fish Conservation Netherlands (RAVON) (Jolianne Rijks presenting)

9:30 – 9:45

Surveillance of ranavirus in frog farms and surrounding environments in Brazil

Rolando Mazzoni
Centro de Pesquisa em Alimentos, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás

9:45 – 10:00

Risk analysis common midwife toad-like virus, the Netherlands

Jolianne Rijks
Dutch Wildlife Health Centre, Faculty of Veterinary Medicine, Utrecht University

10:00 – 10:15

Ranavirus outbreak in captive eastern box turtle (Terrapene carolina carolina) population with mycoplasma and herpesvirus co-infection: Management and monitoring

Richard Sim
Wildlife Center of Virginia


Moderator: Matt Gray


10:45 – 11:15

Ecology and Epidemiology Overview

Matthew Gray
Center for Wildlife Health, University of Tennessee, Knoxville

11:15 – 11:30

Mathematical Modeling of Ranavirus Ecology

Amanda Duffus
Department of Biology, Gordon State College

11:30 – 11:45

Environmental dependency of ranavirus/amphibian genotypic interactions: A coevolutionary rubik’s cube

David Lesbarrères
Genetics and Ecology of Amphibians Research Group (GEARG), Department of Biology, Laurentian University

11:45 – 12:00

The within-pond epidemiology of an amphibian ranavirus

Jesse Brunner
School of Biological Sciences, Washington State University

12:00 – 12:15

Amphibian ranavirus disease dynamics in an industrially altered landscape

Danna Schock
Keyano College

12:15 – 13:45


13:45 – 14:00

Transmission of ranavirus between ectothermic vertebrate hosts

Roberto Brenes
Center for Wildlife Health, University of Tennessee, Knoxville

14:00 – 14:15

Stress effects on susceptibility and transmission of ranavirus infection in amphibians

Robin Warne
Department of Zoology, Southern Illinois University

14:15 – 14:30

Temperature affects anuran susceptibility to ranavirus

Mabre Brand
Center for Wildlife Health and College of Veterinary Medicine, University of Tennessee, Knoxville


Poster Presentations


1.      Balsiero, A., C. Mavián, A. López-Bueno, A. Alcamí, A. Alejo, D. Miller, and R. Casais. Common midwife toad virus: Outbreaks in Europe, pathology and genomic analyses.


2.      Balsiero, A., R. Casais, and D. Miller. Immunohistochemical staining for Ranaviruses.


3.      Brenes, R., J. Hoverman, D. Miller, N. Haislip, S. Roon, and M. Gray. Phylogenetic and life history traits correlated with susceptibility to ranavirus: An expanded analysis with 35 species.


4.      Brenes, R., Gray, M., R. Wilkes, and D. Miller. Presence of amplification hosts increases mortality of syntopic amphibians by ranaviral disease.


5.      Chaney, J., M. Gray, R. Wilkes and D. Miller. Ranavirus is lethal to boreal toad (Bufo boreas) tadpoles.


6.      Chen, Z.-Y., J-F. Gui, X-C. Gao, C. Pei, Y-J. Hong,and Q-Y. Zhang. Genome annotation of a ranavirus (ADRV) associated with high mortality in Chinese giant salamander and cross-species transmission.


7.      Cheng, E., M. Jones, J. Jancovich, J. Burchell, D. Reavill, D. Imai, A. Urban, L. Jackson, M. Kirkendall, V. Chinchar, and A. Pessier. Identification of a Bohle iridovirus-like agent in captive North American boreal toads.


8.      Densmore, C. Susceptibility to the tadpole edema virus among early life stages of the Fowler’s toad (Anaxyrus fowleri)


9.      Duffus, A. and S. Jackson-Rosario. Iridovirus phylogenetics: Is the major capsid protein enough?


10.  Goodman, R., F. Polakiewicz, and D. Miller. Effect of herbicides and ranavirus on survival and health of juvenile freshwater turtles.


11.  Greno, L., A. Allen, and J. Jancovich. Insight into the pathogenic determinants of Ambystoma tigrinum virus


12.  Hall, E. and E. Crespi. Susceptibility across the landscape: Integrating epidemiology and host physiology.


13.  Hamel, K., M. Bravo, J. Abrams McLean, T. Waltzek, and J. Jancovich. Genomic sequence of largemouth bass virus.


14.  Hardman, R., W. Sutton, D. McGinnity, K. Irwin, S. Reinsch, B. Fitzpatrick, P. Colclough, M. Souza, M. Freake, M. Gray and D. Miller. Prevalence of ranavirus and Bd in hellbender populations in Tennessee and Arkansas.


15.  He, L.-B., and Q.-Y. Zhang. Construction and analysis of a recombinant frog ranavirus (RGV) containing the lac repressor-operator system.


16.  Huang, Y., X. Huang and Q. Qin. Involvement of MAPK signaling pathway in soft-shelled turtle iridovirus (STIV) induced apoptosis.


17.  Iwanowicz,L.,  C. Densmore, and C. Hahn. Isolation and partial sequencing of a FV3-like ranavirus from the carcass of a juvenile eastern painted turtle (Chysemys picta picta)


18.  Iwanowicz, L., C. Densmore, C. Hahn, P. McAllister, and J. Odenkirk. Identification of largemouth bass virus in the introduced snakehead (Channa argus) inhabiting the Chesapeake Bay watershed.


19.  Johnson, A., and J. Brunner. The effect of community interactions on amphibian ranavirus persistence in aquatic ecosystems.


20.  Kik, M., M. Stege, R. Boonyarittichaikij, and A. van Asten. Concurrent ranavirus and Batrachochytrium dendrobatids infection in captive frogs (Phyllobates and Dendrobates species), the Netherlands, 2012. A first report.


21.  McMillan, K., D. Lesbarreres, T. Garner, and C. Donnelly. Spatial epidemiology of amphibian emerging infectious diseases in Ontario, Canada


22.  Monsen – Collar, K., L. Hazard, and P. Dolcemascolo. A ranavirus-related mortality event and the first report of ranavirus in New Jersey.


23.  Verdan Neves, P., M. Braga Catrox , M. Hipolito, R. Mazzoni, and C. M. Ferreira. Use of the immunoelectronic microscopy technique for the diagnosis of ranavirus in American bullfrogs (Lithobates catesbeianus).


24.  O’Connor, K., and T. Rittenhouse. Ranavirus prevalence in wood frog tadpoles throughout Connecticut.

25.  Olson, D., A. Duffus, M. Gray, D. Miller, and M. Hines. The global ranavirus reporting system.


26.  Price, S., T. Garner, R. Nichols, and J. Bosch. Introduction of diverse ranaviruses to northern Spain: Likely cause of population declines in multiple hosts.


27.  Sifkarovski, J., L. Grayfer, F. De Jesús Andino, B. Lawrence, and J. Robert. Negative effects of below-toxic levels of atrazine on immunity to ranavirus using Xenopus as a novel model system.


28.  Reilly, P., M. Gray, R. Wilkes, J. Chaney, and D. Miller. Does geographic distance between host population and isolate location equate to ranavirus pathogenicity?


29.  Storfer, A., and K. Chojnacki, A bullfrog ranavirus strain isolated from a farm is more virulent that a wild-caught strain.


30.  Sutton, W., M. Gray, J. Hoverman, R. Secrist, P., Super, R. Hardman, J. Tucker, and D. Miller. Trends in ranavirus prevalence among Plethodotid salamanders in the Great Smoky Mountains National Park.


31.  Sutton, W., M. Gray, R. Hardman, and D. Miller. High susceptibility of the most endangered frog in North America (Rana sevosa) to ranavirus.


32.  Tucker, J., R. Brenes, D. Miller, T. Waltzek, R. Wilkes, M. Brand, J. Chaney, R. Hardman, N. Hilzinger, R. Huether, and M. Gray. Susceptibility of common fish and chelonians to ranavirus.


33.  Une, Y., E. Ri, M. Katou, and M. Murakami. Outbreak of ranaviral disease in amphibian colonies in Japan.


34.  Winzeler, M., S. Kimblem and R. Williams. Prevalence of ranavirus across Indiana.


35.  Wellehan, Jr., J., and T. Waltzek. Effect of liposome encapsulated cidofovir on frog virus 3 in cell culture.


36.  Yan, Y., H. Cui, S. Jiang, Y. Huang, X. Huang, S. Wei, W. Xu, and Q. Qin. Identification of a novel marine fish virus, Singapore grouper iridovirus-encoded microRNAs expressed in grouper cells by Solexa sequencing.



Global Ranavirus Consortium


UT Department of Forestry, Wildlife and Fisheries