2013 International Symposium on Ranaviruses

Knoxville, Tennessee, USA

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Presentation Slides

Saturday, 27 July 2013

Moderator: Trent Garner

8:30 – 9:30	Keynote Address Richard Speare Emeritus Professor, School of Public Health, James Cook University, Australia
9:30 – 10:00	Emergence and Conservation Overview Trenton Garner Institute of Zoology, Zoological Society of London
10:00 – 10:15	Ranavirus could potentially speed up extinction for the endangered frog (Rana sevosa) Julia EarlNational Institute for Mathematical and Biological Synthesis, University of Tennessee
10:15 – 10:30	Repeated detection of frog virus 3 (FV3) during aquaculture health surveys Thomas Waltzek College of Veterinary Medicine, University of Florida
10:45 – 11:00	Concurrent die-offs of turtles, salamanders and frogs at one site in Maryland, USA Scott Farnsworth School of Biological Sciences, Washington State University
11:00 – 11:15	Distribution of ranaviruses in Japan Yumi Une Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University
11:15 – 11:30	Ranavirus infection in Costa Rican amphibians Jacob Kerby Department of Biology, University of South Dakota
11:30 – 11:45	Characterization of amphibian ranavirus in the international wildlife trade Kristine Smith EcoHealth Alliance

Moderator: Debra Miller

13:30 – 14:00	Pathology and Physiology Overview Debra Miller College of Veterinary Medicine and Center for Wildlife Health, University of Tennessee, Knoxville
14:00 – 14:15	Frog virus 3 in eastern box turtles: Agents seen with coinfections James Wellehan College of Veterinary Medicine, University of Florida
14:15– 14:30	Ranavirus associated dermatitis in lizards Anke Stöhr Institute of Environmental and Animal Hygiene, Universität Hohenheim
14:30 – 14:45	The wood frog, Rana sylvatica (Lithobates sylvaticus), as a model to study the pathogenesis and host-pathogen interactions of frog virus 3 (FV3) Maria Forzán Canadian Cooperative Wildlife Health Centre and Department of Pathology and Microbiology, Atlantic Veterinary College
14:45 – 15:00	Ranaviruses in snakes, lizards and chelonians Rachel Marschang Institute of Environmental and Animal Hygiene, Universität Hohenheim

Moderator: Jacques Robert

15:15 – 15:45	Virology and Immunology Overview Jacques Robert Microbiology & Immunology, University of Rochester Medical Center
15:45 – 16:00	The three dimensional structure and morphogenesis of Singapore grouper iridovirus Jinlu Wu Mechanobiology Institute Singapore, National University of Singapore, Singapore
16:00 – 16:15	Experimental challenge study of ranavirus infection in previously infected eastern box turtles (Terrapene carolina carolina) to assess immunity Jennifer Hausmann Medical Department, Maryland Zoo
16:15 – 16:30	Immune response in fathead minnow cells following infection with frog virus 3 V. Gregory Chinchar Department of Microbiology, University of Mississippi Medical Center
16:30 – 16.:45	Signapore grouper iridovirus (SGIV) induced parapoptosis-like death in host cells via the activation of MAPK signaling Qiwei Qin Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences

Sunday, 28 July 2013

Moderator: Allan Pessier

8:30 – 9:00	Diagnosis, Treatment and Management Overview Allan Pessier Amphibian Disease Laboratory, Institute for Conservation Research, San Diego Zoo
9:00 – 9:15	Chelonian diagnostics, pathology, and therapy Matthew Allender Department of Comparative Biosciences, , College of Veterinary Medicine, University of Illinois
9:15 – 9:30	Monitoring ranavirus-associated mortality in Dutch heathland in the aftermath of an outbreak Annemarieke Spitzen Reptile, Amphibian & Fish Conservation Netherlands (RAVON) (Jolianne Rijks presenting)
9:30 – 9:45	Surveillance of ranavirus in frog farms and surrounding environments in Brazil Rolando Mazzoni Centro de Pesquisa em Alimentos, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás
9:45 – 10:00	Risk analysis common midwife toad-like virus, the Netherlands Jolianne Rijks Dutch Wildlife Health Centre, Faculty of Veterinary Medicine, Utrecht University
10:00 – 10:15	Ranavirus outbreak in captive eastern box turtle (Terrapene carolina carolina) population with mycoplasma and herpesvirus co-infection: Management and monitoring Richard Sim Wildlife Center of Virginia

Moderator: Matt Gray

10:45 – 11:15	Ecology and Epidemiology Overview Matthew Gray Center for Wildlife Health, University of Tennessee, Knoxville
11:15 – 11:30	Mathematical Modeling of Ranavirus Ecology Amanda Duffus Department of Biology, Gordon State College
11:30 – 11:45	Environmental dependency of ranavirus/amphibian genotypic interactions: A coevolutionary rubik’s cube David Lesbarrères Genetics and Ecology of Amphibians Research Group (GEARG), Department of Biology, Laurentian University
11:45 – 12:00	The within-pond epidemiology of an amphibian ranavirus Jesse Brunner School of Biological Sciences, Washington State University
12:00 – 12:15	Amphibian ranavirus disease dynamics in an industrially altered landscape Danna Schock Keyano College
12:15 – 13:45	Lunch
13:45 – 14:00	Transmission of ranavirus between ectothermic vertebrate hosts Roberto Brenes Center for Wildlife Health, University of Tennessee, Knoxville
14:00 – 14:15	Stress effects on susceptibility and transmission of ranavirus infection in amphibians Robin Warne Department of Zoology, Southern Illinois University
14:15 – 14:30	Temperature affects anuran susceptibility to ranavirus Mabre Brand Center for Wildlife Health and College of Veterinary Medicine, University of Tennessee, Knoxville

Poster Presentations

1. Balsiero, A., C. Mavián, A. López-Bueno, A. Alcamí, A. Alejo, D. Miller, and R. Casais. Common midwife toad virus: Outbreaks in Europe, pathology and genomic analyses.

2. Balsiero, A., R. Casais, and D. Miller. Immunohistochemical staining for Ranaviruses.

3. Brenes, R., J. Hoverman, D. Miller, N. Haislip, S. Roon, and M. Gray. Phylogenetic and life history traits correlated with susceptibility to ranavirus: An expanded analysis with 35 species.

4. Brenes, R., Gray, M., R. Wilkes, and D. Miller. Presence of amplification hosts increases mortality of syntopic amphibians by ranaviral disease.

5. Chaney, J., M. Gray, R. Wilkes and D. Miller. Ranavirus is lethal to boreal toad (Bufo boreas) tadpoles.

6. Chen, Z.-Y., J-F. Gui, X-C. Gao, C. Pei, Y-J. Hong,and Q-Y. Zhang. Genome annotation of a ranavirus (ADRV) associated with high mortality in Chinese giant salamander and cross-species transmission.

7. Cheng, E., M. Jones, J. Jancovich, J. Burchell, D. Reavill, D. Imai, A. Urban, L. Jackson, M. Kirkendall, V. Chinchar, and A. Pessier. Identification of a Bohle iridovirus-like agent in captive North American boreal toads.

8. Densmore, C. Susceptibility to the tadpole edema virus among early life stages of the Fowler’s toad (Anaxyrus fowleri)

9. Duffus, A. and S. Jackson-Rosario. Iridovirus phylogenetics: Is the major capsid protein enough?

10. Goodman, R., F. Polakiewicz, and D. Miller. Effect of herbicides and ranavirus on survival and health of juvenile freshwater turtles.

11. Greno, L., A. Allen, and J. Jancovich. Insight into the pathogenic determinants of Ambystoma tigrinum virus

12. Hall, E. and E. Crespi. Susceptibility across the landscape: Integrating epidemiology and host physiology.

13. Hamel, K., M. Bravo, J. Abrams McLean, T. Waltzek, and J. Jancovich. Genomic sequence of largemouth bass virus.

14. Hardman, R., W. Sutton, D. McGinnity, K. Irwin, S. Reinsch, B. Fitzpatrick, P. Colclough, M. Souza, M. Freake, M. Gray and D. Miller. Prevalence of ranavirus and Bd in hellbender populations in Tennessee and Arkansas.

15. He, L.-B., and Q.-Y. Zhang. Construction and analysis of a recombinant frog ranavirus (RGV) containing the lac repressor-operator system.

16. Huang, Y., X. Huang and Q. Qin. Involvement of MAPK signaling pathway in soft-shelled turtle iridovirus (STIV) induced apoptosis.

17. Iwanowicz,L., C. Densmore, and C. Hahn. Isolation and partial sequencing of a FV3-like ranavirus from the carcass of a juvenile eastern painted turtle (Chysemys picta picta)

18. Iwanowicz, L., C. Densmore, C. Hahn, P. McAllister, and J. Odenkirk. Identification of largemouth bass virus in the introduced snakehead (Channa argus) inhabiting the Chesapeake Bay watershed.

19. Johnson, A., and J. Brunner. The effect of community interactions on amphibian ranavirus persistence in aquatic ecosystems.

20. Kik, M., M. Stege, R. Boonyarittichaikij, and A. van Asten. Concurrent ranavirus and Batrachochytrium dendrobatids infection in captive frogs (Phyllobates and Dendrobates species), the Netherlands, 2012. A first report.

21. McMillan, K., D. Lesbarreres, T. Garner, and C. Donnelly. Spatial epidemiology of amphibian emerging infectious diseases in Ontario, Canada

22. Monsen – Collar, K., L. Hazard, and P. Dolcemascolo. A ranavirus-related mortality event and the first report of ranavirus in New Jersey.

23. Verdan Neves, P., M. Braga Catrox , M. Hipolito, R. Mazzoni, and C. M. Ferreira. Use of the immunoelectronic microscopy technique for the diagnosis of ranavirus in American bullfrogs (Lithobates catesbeianus).

24. O’Connor, K., and T. Rittenhouse. Ranavirus prevalence in wood frog tadpoles throughout Connecticut.

25. Olson, D., A. Duffus, M. Gray, D. Miller, and M. Hines. The global ranavirus reporting system.

26. Price, S., T. Garner, R. Nichols, and J. Bosch. Introduction of diverse ranaviruses to northern Spain: Likely cause of population declines in multiple hosts.

27. Sifkarovski, J., L. Grayfer, F. De Jesús Andino, B. Lawrence, and J. Robert. Negative effects of below-toxic levels of atrazine on immunity to ranavirus using Xenopus as a novel model system.

28. Reilly, P., M. Gray, R. Wilkes, J. Chaney, and D. Miller. Does geographic distance between host population and isolate location equate to ranavirus pathogenicity?

29. Storfer, A., and K. Chojnacki, A bullfrog ranavirus strain isolated from a farm is more virulent that a wild-caught strain.

30. Sutton, W., M. Gray, J. Hoverman, R. Secrist, P., Super, R. Hardman, J. Tucker, and D. Miller. Trends in ranavirus prevalence among Plethodotid salamanders in the Great Smoky Mountains National Park.

31. Sutton, W., M. Gray, R. Hardman, and D. Miller. High susceptibility of the most endangered frog in North America (Rana sevosa) to ranavirus.

32. Tucker, J., R. Brenes, D. Miller, T. Waltzek, R. Wilkes, M. Brand, J. Chaney, R. Hardman, N. Hilzinger, R. Huether, and M. Gray. Susceptibility of common fish and chelonians to ranavirus.

33. Une, Y., E. Ri, M. Katou, and M. Murakami. Outbreak of ranaviral disease in amphibian colonies in Japan.

34. Winzeler, M., S. Kimblem and R. Williams. Prevalence of ranavirus across Indiana.

35. Wellehan, Jr., J., and T. Waltzek. Effect of liposome encapsulated cidofovir on frog virus 3 in cell culture.

36. Yan, Y., H. Cui, S. Jiang, Y. Huang, X. Huang, S. Wei, W. Xu, and Q. Qin. Identification of a novel marine fish virus, Singapore grouper iridovirus-encoded microRNAs expressed in grouper cells by Solexa sequencing.

Global Ranavirus Consortium

UT Department of Forestry, Wildlife and Fisheries

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